The One Health 4 Cambodia (OH4CAM) project aims to establish a comprehensive and sustainable One Health (OH) framework in Cambodia to address antimicrobial resistance (AMR), zoonotic diseases (ZDs), and emerging infectious diseases (EIDs) in Cambodia. The OH4CAM project approach involves coordination and collaboration across public health, veterinary medicine, and environmental management for health system strengthening and disease surveillance and prevention. Cambodia is uniquely positioned to serve as a demonstration of OH success, with strong commitment from the Royal Cambodian Government. The One Health Inter-Ministerial Coordinating Committee (IMCC-OH) represents a significant step forward in Cambodia's approach to foster collaboration between various ministries and stakeholders to tackle the above-mentioned human, animal and environmental challenges, which are currently emerging in the country and globally. The OH4CAM project, funded by KfW in the form of a EUR 34 million investment package over a 5-year period, will assist the three Ministry of Health (MOH), Ministry of Agriculture, Forestry and Fisheries (MAFF), and Ministry of Environment (MOE) to identify prioritized interventions in Cambodia. This architecture aims to establish and implement a comprehensive, sustainable, operational, and effective OH framework in Cambodia, demonstrating readiness to respond to AMR, ZDs, and EIDs. The Center for Health and Social Development (HSD) is a Cambodian non-profit organization established in 2012 to expand access to high-quality health and social interventions. HSD implements various health research and service delivery projects, including those related to malaria, TB, and One Health covering biosafety, and biosecurity. These projects are funded by various donors.